Diverse control mechanisms converge to ensure that gene transcripts are expressed and processed accurately. Dissection of these interactions has proven challenging, because most experimental approaches record downstream products fed by multiple pathways –...
Repair of Double stranded DNA breaks-pathway choices and more
Double stranded DNA breaks (DSBs) are critical for cell health as a single unrepaired DSB is sufficient for inducing apoptosis. Two major mechanistically distinct pathways, homologous...
Dr. Chen's group has developed a number of novel conditional mouse strains that have aided in their detailed characterization and elucidation of the molecular mechanisms of epsin-dependent dysfunctions in heart disease, diabetes, obesity and cancer, using...
Cells in complex mammalian tissues, such as the brain, are spatially organized and dynamic, yet almost all genomic tools lack temporal and spatial resolution. Our laboratory, at the Broad institute, sets out to build a set of tools which will bridge...
Hematopoiesis serves as a useful model system for studies of cell fate determination and lineage plasticity. Lineage-specific transcription factors play essential roles in these processes by activating terminal maturation genes, while simultaneously...
Our lab studies eukaryotic gene expression. We are concentrating on three areas: (A) the functions and interactions of the RNA polymerase II (RNApII) transcription factors, (B) communication between chromatin and transcription complexes, particularly...
We are interested in understanding transport mechanisms in the cell. In particular, we are interested in how molecules are transported into, out of, and within cilia. Cilia (also known as flagella) are finger-like organelles that project from the surface...
The long-term goal of our research program is to understand the molecular logic of signal transduction, focusing on proteins implicated in human disease. The laboratory currently emphasizes structure-function studies in Notch signaling and maintains an...
Bacterial cells come in a wide variety of shapes and sizes. Within a given class, however, cells typically grow and divide in such a way as to maintain the particular shape and size distribution programmed by their genome. Research in my laboratory is...
Therapeutic Genome Editing
The β-hemoglobinopathies comprise the most common monogenic diseases worldwide, characterized by mutations of the β-globin constituent of hemoglobin. Re-induction of fetal hemoglobin (HbF) would counteract the fundamental...
